WSCD2 Mouse Monoclonal Antibody [9-F3]
cat.: M1010-2
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 9-F3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein L affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein with Human WSCD2 50-353 / 565. |
| Positive control: | WSCD2 recombinant protein lysates, mouse hippocampus tissue lysate, rat brain tissue lysate, SH-SY5Y, human thyroid tissue, human liver cancer tissue, human uterus tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: Q2TBF2 Human | D4PHA7 Mouse Entrez Gene: 360824 Rat |
| Alternative names: | 4933413A10Rik C530024P05Rik Gm450 WSC domain containing 2 WSC domain containing protein 2 WSC domain-containing protein 2 WSCD 2 WSCD2 WSCD2_HUMAN |
Images
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Fig1: Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution. |
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Fig2:
Western blot analysis of WSCD2 on different lysates with Mouse anti-WSCD2 antibody (M1010-2) at 1/500 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1010-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining WSCD2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-WSCD2 antibody. Counter stained with |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-WSCD2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-WSCD2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.