GRAMD1A Mouse Monoclonal Antibody [1-E4]
cat.: M1010-3
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 1-E4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within Human GRAMD1A aa 1-724 / 724. |
| Positive control: | NIH/3T3 cells lysates, SHG-44 cells lysates, A549, human liver tissue, human liver carcinoma tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle, autophagosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:400 |
| Uniprot #: | SwissProt: Q96CP6 Human |
Images
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Fig1: Western blot analysis of GRAMD1A on NIH/3T3 (1) and SHG-44 (2) cells lysates using anti-GRAMD1A antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of A549 cells labeling GRAMD1A with Mouse anti-GRAMD1A antibody (M1010-3) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRAMD1A antibody (M1010-3) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRAMD1A antibody (M1010-3) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1010-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-GRAMD1A antibody (M1010-3) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1010-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.