PGBD5 Mouse Monoclonal Antibody [7-F8-5]
cat.: M1012-1
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: 7-F8-5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 58 kDa
Isotype: IgG1
Immunogen: Synthetic peptide with Human PGBD5 aa 61-110 / 524.
Positive control: Human brain tissue lysate, Mouse brain tissue lysate, SH-SY5Y, Neuro-2a, C6, human lung squamous cell carcinoma tissue, mouse hippocampus tissue, mouse cerebellum tissue, rat cerebellum tissue.
Subcellular location: Nucleus
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:100-1:1,000
1:200-1:400
Uniprot #: SwissProt: Q8N414 Human | D3YZI9 Mouse
Entrez Gene: 292098 Rat
Alternative names: PGBD5 PGBD5_HUMAN PiggyBac transposable element-derived protein 5
Images
M1012-1_1.jpg Fig1: Western blot analysis of PGBD5 on different lysates with Mouse anti-PGBD5 antibody (M1012-1) at 1/500 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 58 kDa
Observed band size: 55 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1012-1) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
M1012-1_2.jpg Fig2: Immunocytochemistry analysis of SH-SY5Y cells labeling PGBD5 with Mouse anti-PGBD5 antibody (M1012-1) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGBD5 antibody (M1012-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
M1012-1_3.jpg Fig3: Immunocytochemistry analysis of Neuro-2a cells labeling PGBD5 with Mouse anti-PGBD5 antibody (M1012-1) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGBD5 antibody (M1012-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
M1012-1_4.jpg Fig4: Immunocytochemistry analysis of C6 cells labeling PGBD5 with Mouse anti-PGBD5 antibody (M1012-1) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PGBD5 antibody (M1012-1) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
M1012-1_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1012-1_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1012-1_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1012-1_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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