PGBD5 Mouse Monoclonal Antibody [7-F8-5]
cat.: M1012-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7-F8-5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide with Human PGBD5 aa 61-110 / 524. |
| Positive control: | Human brain tissue lysate, mouse brain tissue lysate, F9, human lung squamous cell carcinoma tissue, mouse hippocampus tissue, mouse cerebellum tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:100-1:500 1:100-1:400 |
| Uniprot #: | SwissProt: Q8N414 Human | D3YZI9 Mouse |
| Alternative names: | PGBD5 PGBD5_HUMAN PiggyBac transposable element-derived protein 5 |
Images
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Fig1:
Western blot analysis of PGBD5 on different lysates with Mouse anti-PGBD5 antibody (M1012-1) at 1/500 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1012-1) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunofluorescent staining of F9 cells using anti-PGBD5 Mouse mAb (Cat. # M1012-1). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-PGBD5 antibody (M1012-1) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1012-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.