C10orf58 Mouse Monoclonal Antibody [7-B7]
cat.: M1012-4
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 7-B7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human C10orf58 aa 1-229 / 229. |
| Positive control: | Recombinant protein, human skin tissue lysate, HepG2, HUVEC, PC-3M. |
| Subcellular location: | Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q9BRX8 Human |
| Alternative names: | C10orf58 CJ058_HUMAN UPF0765 protein C10orf58 |
Images
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Fig1: ICC staining C10orf58 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining C10orf58 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining C10orf58 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (M1012-4) at 1/2,000 dilution. Lane 1: HUVEC (Human umbilical vein endothelial cell) cell lysate Lane 2: SK-MEL-28 (Human melanoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 106 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1012-4, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Predicted band size: 25 kDa Observed band size: 25 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.