LRRN3 Mouse Monoclonal Antibody [A4-6-3]
cat.: M1012-8
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A4-6-3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 79 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within human LRRN3 aa 1-260. |
| Positive control: | SHSY5Ycell lysate, rat testis tissue lysate, F9. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H3W5 Human | Q8CBC6 Mouse | Q9ESY6 Rat |
| Alternative names: | Leucine-rich repeat neuronal protein 3L antibidy Neuronal leucine-rich repeat protein 3 antibidy NLRR-3 antibidy Nbla10363 antibidy UNQ194 antibidy LRRN3 antibidy PRO220 antibidy |
Images
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Fig1:
Western blot analysis of LRRN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1012-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY5Ycell lysate Lane 2: Rat testis tissue lysate |
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Fig2: ICC staining of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1012-8, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.