LRRN3 Mouse Monoclonal Antibody [A4-6-3]
cat.: M1012-8
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A4-6-3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 79 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within human LRRN3 aa 1-260. |
| Positive control: | SHSY5Ycell lysate, Pig brain tissue lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, Rat testis tissue lysate, Rat brain tissue lysate, F9. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H3W5 Human | Q8CBC6 Mouse | Q9ESY6 Rat |
| Alternative names: | Leucine-rich repeat neuronal protein 3L antibidy Neuronal leucine-rich repeat protein 3 antibidy NLRR-3 antibidy Nbla10363 antibidy UNQ194 antibidy LRRN3 antibidy PRO220 antibidy |
Images
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Fig1:
Western blot analysis of LRRN3 on different lysates with Mouse anti-LRRN3 antibody (M1012-8) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate, 15 µg/lane Lane 2: Pig brain tissue lysate, 30 µg/lane Lane 3: Mouse testis tissue lysate, 30 µg/lane Lane 4: Mouse brain tissue lysate, 30 µg/lane Lane 5: Rat testis tissue lysate, 30 µg/lane Lane 6: Rat brain tissue lysate, 30 µg/lane Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1012-8, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 79.5 kDa Observed band size: 79 kDa |
|
Fig2: ICC staining of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1012-8, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.