DNA-PKcs/PRKDC Mouse Monoclonal Antibody [2-B3]
cat.: M1204-6
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 2-B3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 469 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Mouse DNA-PKcs/PRKDC aa 3,900-4,128/4,128. |
| Positive control: | K-562 cell lysate, HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, HT-29 cell lysate, A549 cell lysate, MCF7 cell lysate, HepG2, human breast carcinoma tissue, human prostate carcinoma tissue, human colon cancer tissue. |
| Subcellular location: | Nucleus, nucleolus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:100 1:500 |
| Uniprot #: | SwissProt: P78527 Human | P97313 Mouse |
| Alternative names: | DNA dependent protein kinase catalytic subunit DNA PK catalytic subunit DNA-dependent protein kinase catalytic subunit DNA-PK catalytic subunit DNA-PKcs DNAPK DNAPK catalytic subunit DNPK 1 DNPK1 Hyper radiosensitivity of murine scid mutation, complementing 1 Hyperradiosensitivity complementing 1, mouse, homolog of 1 HYRC 1 HYRC HYRC1 IMD26 p350 p460 PKRDC PRKDC PRKDC_HUMAN Protein Kinase DNA Activated Catalytic Polypeptide XRCC 7 XRCC7 |
Images
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Fig1:
Western blot analysis of DNA-PKcs/PRKDC on different lysates with Mouse anti-DNA-PKcs/PRKDC antibody (M1204-6) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lane 6: HT-29 cell lysate Lane 7: A549 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 469 kDa Observed band size: 469 kDa Exposure time: 23 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1204-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling DNA-PKcs/PRKDC with Rabbit anti-DNA-PKcs/PRKDC antibody (M1204-6) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DNA-PKcs/PRKDC antibody (M1204-6) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-DNA-PKcs/PRKDC antibody (M1204-6) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1204-6) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Mouse anti-DNA-PKcs/PRKDC antibody (M1204-6) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1204-6) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-DNA-PKcs/PRKDC antibody (M1204-6) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1204-6) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.