Bcl-2 Mouse Monoclonal Antibody [F7-1-A1]
cat.: M1206-4
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | F7-1-A1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human BCL-2 aa 1-200. |
| Positive control: | Jurkat cell lysate, rat spleen tissue lysate, HepG2, human breast carcinoma tissue, mouse spleen tissue. |
| Subcellular location: | Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:500 1:200 |
| Uniprot #: | SwissProt: P10415 Human | P10417 Mouse | P49950 Rat |
| Alternative names: | Apoptosis regulator Bcl 2 Apoptosis regulator Bcl-2 Apoptosis regulator Bcl2 AW986256 B cell CLL/lymphoma 2 B cell leukemia/lymphoma 2 Bcl-2 Bcl2 BCL2_HUMAN C430015F12Rik D630044D05Rik D830018M01Rik Leukemia/lymphoma, B-cell, 2 Oncogene B-cell leukemia 2 PPP1R50 Protein phosphatase 1, regulatory subunit 50 Bcl 2 |
Images
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Fig1: Western blot analysis of BCL-2 on Jurkat cell lysate using anti-BCL-2 antibody at 1/100 dilution. |
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Fig2:
Western blot analysis of Bcl-2 on rat spleen tissue lysates with Mouse anti-Bcl-2 antibody (M1206-4) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1206-4) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunofluorescent staining of HepG2 cells using anti-Bcl-2 Mouse mAb (Cat. # M1206-4). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BCL-2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-BCL-2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.