HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody [1-14]
cat.: M1208-2
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Rabbit
Applications: WB, ELISA
Clonality: Monoclonal
Clone number: 1-14
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 25 kDa
Isotype: IgG1
Immunogen: Purified Rabbit IgG κ light chain
Positive control: Rabbit Serum
Subcellular location: secrete
Recommended Dilutions:
  WB
  ELISA

1:5,000
1:5,000
Images
M1208-2_1.jpg Fig1: Western blot analysis of Rabbit IgG kappa light chain on different lysates with Mouse anti-Rabbit IgG kappa light chain antibody (M1208-2) at 1/1,000 dilution.

Lane 1: Jurkat (Human T-lymphocyte leukemia cells) cell lysate

Exposure time: 59 seconds; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: CD168(HA721775) 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, 2 hour at room temperatur
Secondary antibody: M1208-2, in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 25.0 kDa
Observed band size: 25 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.