| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | 1-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified Rabbit IgG κ light chain |
| Positive control: | Rabbit Serum |
| Subcellular location: | secrete |
| Recommended Dilutions:
WB ELISA |
1:5,000 1:5,000 |
|
Fig1:
Western blot analysis of Rabbit IgG kappa light chain on different lysates with Mouse anti-Rabbit IgG kappa light chain antibody (M1208-2) at 1/1,000 dilution. Lane 1: Jurkat (Human T-lymphocyte leukemia cells) cell lysate Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: CD168(HA721775) 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, 2 hour at room temperatur Secondary antibody: M1208-2, in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25.0 kDa Observed band size: 25 kDa |