GFP Mouse Monoclonal Antibody [A11-C11]
cat.: M1210-1
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IF-Cell, IHC-P, IP, WB |
| Clonality: | Monoclonal |
| Clone number: | A11-C11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant full length protein of Aequorea victoria GFP. |
| Positive control: | K562 cells transfected with GFP |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:1000 1:500-1:1,000 1:100 2-5 µg/ml. |
| Uniprot #: | SwissProt: P42212 AEQVI |
| Alternative names: | GFP Green fluorescent protein yfp |
Images
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Fig1: Immunofluorescent staining of K562 cells transfected with GFP gene using anti-GFP Mouse mAb (Cat. # M1210-1). |
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Fig2: Immunohistochemical analysis of paraffin- embedded testis tissue of OCT4-IRES-EGFP mouse(B6;129S4-Pou5f1tm2Jae/J) using anti-GFP Mouse mAb (Cat. # M1210-1). |
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Fig3:
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with M1210-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1604-25 at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: M1210-1 IP in GFP Tag fusion protein lysate. Lane 3: EM30501 IP in GFP Tag fusion protein lysate. Lane 4: Mouse IgG instead of M1210-1 in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.