HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
cat.: M1210-5
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A2-F6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within N-terminal human Beta-actin . |
| Positive control: | PC12 cell lysate, Hela cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P60709 Human | P60710 Mouse | P60711 Rat |
| Alternative names: | A26C1A A26C1B ACTB ACTB_HUMAN Actin beta Actin cytoplasmic 1 Actin, cytoplasmic 1, N-terminally processed Actx b actin Beta cytoskeletal actin Beta-actin BRWS1 E430023M04Rik MGC128179 PS1TP5 binding protein 1 PS1TP5BP1 β-actin β actin |
Images
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Fig1:
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (M1210-5) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Rat lung tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat colon tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1210-5, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Predicted band size: 42 kDa Observed band size: 42 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.