HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
cat.: M1210-5
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: A2-F6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: 42 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within N-terminal human Beta-actin .
Positive control: PC12 cell lysate, Hela cell lysate, NIH/3T3 cell lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: P60709 Human | P60710 Mouse | P60711 Rat
Alternative names: A26C1A A26C1B ACTB ACTB_HUMAN Actin beta Actin cytoplasmic 1 Actin, cytoplasmic 1, N-terminally processed Actx b actin Beta cytoskeletal actin Beta-actin BRWS1 E430023M04Rik MGC128179 PS1TP5 binding protein 1 PS1TP5BP1 β-actin β actin
Images
M1210-5_1.jpg Fig1: Western blot analysis of beta Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-5, 1/1,000) was used in 5% BSA at room temperature for 2 hours.
Positive control:
Lane 1: PC12 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.