KIAA0100 Mouse Monoclonal Antibody [A0-F3]
cat.: M1210-6
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A0-F3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 254 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human KIAA0100 aa 1,947-2,235 / 2,235. |
| Positive control: | Hela, human breast cancer tissue, human kidney tissue, human pancreas tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Mitochondrion membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:10,000 1:100-1:500 1:2,000 |
| Uniprot #: | SwissProt: Q14667 Human |
| Alternative names: | Antigen MLAA-22 BCOX BCOX1 Breast cancer-overexpressed gene 1 protein CT101 DKFZp686M0843 K0100_HUMAN KIAA0100 MGC111488 MGC134981 UPF0378 protein KIAA0100 |
Images
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Fig1: Immunofluorescent staining of Hela cells using anti-KIAA0100 Mouse mAb (Cat. # M1210-6). |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-KIAA0100 antibody (M1210-6) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-6) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-KIAA0100 antibody (M1210-6) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-6) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-KIAA0100 antibody (M1210-6) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1210-6) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of KIAA0100 on different lysates with Mouse anti-KIAA0100 antibody (M1210-6) at 1/1,000 dilution. Lane 1: MDA-MB-231(Human triple-negative breast cancer cells) cell lysate Lane 2: SK-Br-3(Human breast cancer cells) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lane 4: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 5: A431 (human epidermal carcinoma cells) cell lysate Lane 6: PC-3M(Human prostate cancer cells) cell lysate Lane 7: LNCaP (Human prostate cancer cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1210-6, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 253.7 kDa Observed band size: 40-50,254 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.