HP1 alpha Mouse Monoclonal Antibody [B0-1]
cat.: M1211-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | B0-1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human HP1 alpha aa 1-191 / 191. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, HeLa, NIH/3T3. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:500 |
| Uniprot #: | SwissProt: P45973 Human | Q61686 Mouse Entrez Gene: 300266 Rat |
| Alternative names: | Antigen p25 CBX5 CBX5_HUMAN CG8409 Chromobox 5 Chromobox homolog 5 (HP1 alpha homolog, Drosophila) Chromobox homolog 5 Chromobox protein homolog 5 Epididymis luminal protein 25 HEL25 Heterochromatin protein 1 alpha Heterochromatin protein 1 Heterochromatin protein 1 homolog alpha HP1 alpha HP1 alpha homolog HP1 HP1A HP1Hs alpha Su(var)205 |
Images
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Fig1:
Western blot analysis of HP1 alpha on different lysates with Mouse anti-HP1 alpha antibody (M1211-2) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: RAW264.7 cell lysate (10 µg/Lane) Lane 5: C6 cell lysate (10 µg/Lane) Lane 6: PC-12 cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: M1211-2, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: NIH/3T3 (Mouse fibroblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: M1211-2, 1/200, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.