CD11b Mouse Monoclonal Antibody [A2-9]
cat.: M1211-6
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | A2-9 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human CD11b 500-750 aa. |
| Positive control: | TF-1 cell lysate, human tonsil tissue, human spleen tissue, Raji. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC IF-Tissue |
1:1,000 1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: P11215 Human |
| Alternative names: | antigen CD11b (p170) Antigen CD11b p170 CD11 antigen like family member B CD11 antigen-like family member B CD11b CD11b/CD18 CD49d Cell surface glycoprotein MAC-1 subunit alpha Complement component 3 receptor 3 subunit Complement Component Receptor 3 Alpha Complement receptor type 3, alpha subunit CR 3 alpha chain (CR3A) CR 3 alpha chain CR-3 alpha chain CR3 CR3A F730045J24Rik Integrin Alpha M Integrin alpha M chain Integrin alpha-M Integrin beta 2 alpha subunit Integrin subunit alpha M integrin, alpha M (complement component 3 receptor 3 subunit) ITAM_HUMAN ITGAM Leukocyte adhesion receptor MO1 Ly-40 MAC 1 Mac-1a MAC1 Mac1, alpha subunit MAC1A Macrophage antigen alpha polypeptide MGC117044 Mo1, alpha subunit MO1A Neutrophil adherence receptor alpha M subunit Neutrophil adherence receptor SLEB6 |
Images
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Fig1:
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Application: IF-Tissue Species: Human Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/200 |
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Fig5: Flow cytometric analysis of Raji cells with CD11b antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.