ID2 Mouse Monoclonal Antibody [A4-D4]
cat.: M1301-2
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A4-D4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein with Human ID2 aa 1-80 / 134. |
| Positive control: | HEK-293 cell lysate, HT-29 cell lysate, SK-OV-3 cell lysate, human pancreatic cancer tissue. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:10,000 1:100 |
| Uniprot #: | SwissProt: Q02363 Human | P41136 Mouse |
| Alternative names: | bHLHb26 Cell growth inhibiting gene 8 class B basic helix loop helix protein 26 Class B basic helix-loop-helix protein 26 DNA binding protein inhibitor ID 2 DNA binding protein inhibitor ID2 DNA-binding protein inhibitor ID-2 GIG 8 GIG8 Helix loop helix protein ID2 ID2 ID2_HUMAN ID2A ID2H Inhibitor of differentiation 2 Inhibitor of DNA binding 2 Inhibitor of DNA binding 2, dominant negative helix loop helix protein MGC26389 |
Images
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Fig1:
Western blot analysis of ID2 on different lysates with Mouse anti-ID2 antibody (M1301-2) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-OV-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1301-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis on recombinant protein using anti-ID2 Mouse mAb (Cat. # M1301-2). |
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Fig3: Immunohistochemical analysis of paraffin- embedded human pancreatic cancer tissue using anti-ID2 Mouse mAb (Cat. # M1301-2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.