Cardiac Troponin T Mouse Monoclonal Antibody [5-C7]
cat.: M1302-1
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 5-C7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within human TNNT2 50-100 aa. |
| Positive control: | Human heart tissue lysate, SK-Br-3, mouse heart tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P45379 Human | P50752 Mouse |
| Alternative names: | Cardiac muscle troponin T Cardiomyopathy dilated 1D (autosomal dominant) Cardiomyopathy hypertrophic 2 CMD1D CMH2 CMPD2 cTnT LVNC6 MGC3889 OTTHUMP00000033864 OTTHUMP00000033865 OTTHUMP00000033866 OTTHUMP00000033867 OTTHUMP00000033870 OTTHUMP00000218095 RCM3 TNNT 2 TNNT2 TNNT2_HUMAN TnTc Troponin T cardiac muscle Troponin T type 2 (cardiac) Troponin T type 2 cardiac Troponin T, cardiac muscle Troponin T2 cardiac |
Images
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Fig1: Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution. |
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Fig2: ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin. |
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Fig4: Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.