TMEM177 Mouse Monoclonal Antibody [A6-A11-9]
cat.: M1306-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A6-A11-9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human TMEM177 aa 1-311 / 311. |
| Positive control: | TMEM177 recombinant protein, HepG2, Hela, MCF-7, human colon tissue, human breast tissue, human stamoch tissue, human uterus tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q53S58 Human |
| Alternative names: | TM177_HUMAN Tmem177 Transmembrane protein 177 |
Images
|
Fig1: Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1,000 dilution. |
|
Fig2: ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining TMEM177 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining TMEM177 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-TMEM177 antibody. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-TMEM177 antibody. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded hman stamoch tissue using anti-TMEM177 antibody. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded human uterus muscle tissue using anti-TMEM177 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.