GATA4 Mouse Monoclonal Antibody [7-4]
cat.: M1306-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7-4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within mouse GATA4 aa 200-400/442. |
| Positive control: | A549 cell lysates, human intestine tissue, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q08369 Mouse |
| Alternative names: | ASD2 GATA 4 GATA binding protein 4 GATA-binding factor 4 gata4 GATA4_HUMAN MGC126629 Transcription factor GATA 4 Transcription factor GATA-4 Transcription factor GATA4 VSD1 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human intestine tissue using anti-GATA4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-GATA4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of GATA4 on different lysates with Mouse anti-GATA4 antibody (M1306-2) at 1/1,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 50 seconds; ECL: K1802 Negative expression of GATA4 protein in K562 cell is consistent with the predicted expression pattern Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1306-2, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 50-55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.