NusA Mouse Monoclonal Antibody [A6-E7]
cat.: M1307-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Escherichia coli |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | A6-E7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full length protein of Escherichia coli NusA. |
| Positive control: | E. coli lysates. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB ELISA |
1:500 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P0AFF6 Escherichia coli |
| Alternative names: | Transcription termination/antitermination protein NusA N utilization substance protein A Transcription termination/antitermination L factor nusA b3169 JW3138 |
Images
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Fig1:
Western blot analysis of NusA on E. coli lysates with Mouse anti-NusA antibody (M1307-1) at 1/500 dilution. Lane 1: E. coli lysate (2 µL/Lane) Lane 2: E. coli lysate (5 µL/Lane) Lane 3: E. coli lysate (10 µL/Lane) Lane 4: E. coli lysate (15 µL/Lane) Predicted band size: 55 kDa Observed band size: 65 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1307-1) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.