Axl Mouse Monoclonal Antibody [C4-A8]
cat.: M1310-5
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | C4-A8 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein with Human UFO aa 10-160 / 894. |
| Positive control: | Recombinant protein lysate, SW480, LOVO. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:200-1:500 |
| Uniprot #: | SwissProt: P30530 Human |
| Alternative names: | Adhesion related kinase AI323647 Ark Axl AXL oncogene AXL receptor tyrosine kinase AXL transforming gene AXL transforming sequence/gene EC 2.7.10.1 JTK11 Oncogene AXL Tyro7 Tyrosine protein kinase receptor UFO Tyrosine-protein kinase receptor UFO UFO UFO_HUMAN |
Images
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Fig1: ICC staining UFO (green) in SW480 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining UFO (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3:
Western blot analysis of Axl on different lysates with Mouse anti-Axl antibody (M1310-5) at 1/2,000 dilution. Lane 1: HUVEC(Human umbilical vein endothelial cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1310-5, 1/2,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 98.3 kDa Observed band size: 150 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.