ATRX Mouse Monoclonal Antibody [2-E7]
cat.: M1311-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 2-E7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 280 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within mouse ATRX aa 2,236-2,416 / 2,476. |
| Positive control: | NIH/3T3, mouse kidney tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
IF-Cell IHC-P |
1:100 1:200 |
| Uniprot #: | SwissProt: Q61687 Mouse |
| Alternative names: | Alpha thalassemia/mental retardation syndrome X linked homolog ATP dependent helicase ATRX ATP-dependent helicase ATRX ATR2 Atrx ATRX_HUMAN DNA dependent ATPase and helicase Helicase 2, X linked MGC2094 MRXHF1 RAD54 RAD54L SFM1 SHS Transcriptional regulator ATRX X linked helicase II X linked nuclear protein X-linked helicase II X-linked nuclear protein XH2 XNP Znf HX Znf-HX |
Images
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Fig1:
Immunocytochemistry analysis of NIH/3T3 cells labeling ATRX with Mouse anti-ATRX antibody (M1311-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ATRX antibody (M1311-2) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- ATRX mouse monoclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.