Lin28B Mouse Monoclonal Antibody [A2-4-5]
cat.: M1312-1
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A2-4-5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human Lin28B aa 73-250 / 250. |
| Positive control: | JAR cell lysate, NCCIT, human placenta tissue. |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q6ZN17 Human |
| Alternative names: | CSDD 2 CSDD2 FLJ16517 Lin 28 homolog B (C. elegans) Lin 28.2 Lin-28B LIN28B LN28B_HUMAN Protein lin-28 homolog B |
Images
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Fig1:
Western blot analysis of Lin28B on different lysates with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (negative) Lane 2: JAR cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1312-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining Lin28B in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. and counterstained with DAPI in order to highlight the nucleus (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-Lin28B antibody (M1312-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1312-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.