p53 Mouse Monoclonal Antibody [C8-A11]
cat.: M1312-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | C8-A11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human p53 aa 344-393. |
| Positive control: | 293 cell lysates, A431, human liver carcinoma tissue. |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
IF-Cell IHC-P WB |
1:200- 1:1,000 1:200 1:500 |
| Uniprot #: | SwissProt: P04637 Human |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
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Fig1: Western blot analysis on 293 cell lysates using anti- p53 mouse mAb. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (M1312-2) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (M1312-2) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti- p53 mouse monoclonal antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.