Cdk2 Mouse Monoclonal Antibody [10-D7]
cat.: M1403-3
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 10-D7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within Human Cdk2 aa 249-298 / 298. |
| Positive control: | A549, F9, PC12, NCCIT |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell |
1:2000 1:500 |
| Uniprot #: | SwissProt: P24941 Human |
| Alternative names: | Cdc2 related protein kinase cdc2-related protein kinase CDC28 CDC2A Cdk 2 CDK1 CDK2 CDK2_HUMAN CDKN2 Cell devision kinase 2 Cell division protein kinase 2 Cyclin dependent kinase 2 cyclin dependent kinase 2-alpha Cyclin-dependent kinase 2 kinase Cdc2 MPF p33 protein kinase p33(CDK2) |
Images
|
Fig1: Western blot analysis on different cell lysates using anti- Cdk2 mouse mAb. |
|
Fig2:
Western blot analysis of Cdk2 on different lysates with Mouse anti-Cdk2 antibody (M1403-3) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Cdk2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: merk 4-20% SDS-PAGE gel. M1403-3 was shown to specifically react with Cdk2 in Hela-si NT cells. Weakened band was observed when Hela-si Cdk2 sample was tested. Hela-si NT and Hela-si Cdk2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (M1403-3, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-mouse IgG-HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3: ICC staining Cdk2 in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.