E-cadherin Mouse Monoclonal Antibody [3-F9]
cat.: M1405-3
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 3-F9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | ~130kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within mouse E-Cadherin aa 350-550. |
| Positive control: | Human liver tissue, mouse liver tissue. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
IHC-P |
1:200 |
| Uniprot #: | SwissProt: P12830 Human | P09803 Mouse |
| Alternative names: | Arc 1 CADH1_HUMAN Cadherin 1 cadherin 1 type 1 E-cadherin Cadherin1 CAM 120/80 CD 324 CD324 CD324 antigen cdh1 CDHE E-Cad/CTF3 E-cadherin ECAD Epithelial cadherin epithelial calcium dependant adhesion protein LCAM Liver cell adhesion molecule UVO Uvomorulin |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-cadherin antibody (M1405-3) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1405-3) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-E-cadherin antibody (M1405-3) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1405-3) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.