| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | B0-5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 44kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human DNMT3L aa 1-250/386. |
| Positive control: | HepG2, NCCIT, mouse colon |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
IF-Cell IHC-P |
1:200-1:500 1:200 |
| Uniprot #: | SwissProt: Q9UJW3 Human |
| Alternative names: | Cytosine 5 methyltransferase 3 like protein DNA (cytosine 5 ) methyltransferase 3 like DNA (cytosine-5)-methyltransferase 3-like DNA cytosine 5 methyltransferase 3 like protein DNA methyltransferase 3 like protein DNM3L_HUMAN Dnmt 3L Dnmt3l Human cytosine 5 methyltransferase 3 like protein MGC1090 |
|
Fig1: ICC staining DNMT3L in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
|
Fig2: ICC staining DNMT3L in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colonic tissue using anti- DNMT3L mouse monoclonal antibody. |