PCNA Mouse Monoclonal Antibody [4-E11]
cat.: M1405-8
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 4-E11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | ~30kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human PCNA aa 31-80 / 261. |
| Positive control: | Raji |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P12004 Human |
| Alternative names: | ATLD2 cb16 Cyclin DNA polymerase delta auxiliary protein etID36690.10 fa28e03 fb36g03 HGCN8729 MGC8367 Mutagen-sensitive 209 protein OTTHUMP00000030189 OTTHUMP00000030190 PCNA Pcna/cyclin PCNA_HUMAN PCNAR Polymerase delta accessory protein Proliferating cell nuclear antigen wu:fa28e03 wu:fb36g04 |
Images
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Fig1:
Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (M1405-8) at 1/1,000 dilution. Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: C2C12 (Mouse myoblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minutes 34 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1405-8, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28.8 kDa Observed band size: 29-36kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.