STAT1 Mouse Monoclonal Antibody [G3-B11]
cat.: M1407-1
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | G3-B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87/83 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human STAT1 aa 71-270 / 750. |
| Positive control: | Jurkat cell lysate, A431 cell lysate, HeLa cell lysate, A549 cell lysate, SK-Br-3 cell lysate, SK-MEL-28 cell lysate, MCF7 cell lysate, HT-29 cell lysate, HT-29, human breast cancer tissue, human spleen tissue. |
| Subcellular location: | Cytoplasm, nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:1,000-1:2,000 1:100-1:200 1:1,000 1:200 |
| Uniprot #: | SwissProt: P42224 Human |
| Alternative names: | Signal transducer and activator of transcription 1 91kD CANDF7 DKFZp686B04100 IMD31A IMD31B IMD31C ISGF 3 ISGF-3 OTTHUMP00000163552 OTTHUMP00000165046 OTTHUMP00000165047 OTTHUMP00000205845 Signal transducer and activator of transcription 1 91kDa Signal transducer and activator of transcription 1 Signal transducer and activator of transcription 1, 91kD Signal transducer and activator of transcription 1-alpha/beta STAT 1 Stat1 STAT1_HUMAN STAT91 Transcription factor ISGF 3 components p91 p84 Transcription factor ISGF-3 components p91/p84 |
Images
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Fig1:
Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (M1407-1) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: MCF7 cell lysate Lane 8: HT-29 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 87/83 kDa Observed band size: 87/83 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1407-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HT-29 cells labeling STAT1 with Mouse anti-STAT1 antibody (M1407-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-STAT1 antibody (M1407-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-STAT1 antibody (M1407-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1407-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-STAT1 antibody (M1407-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1407-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling STAT1 with Rabbit anti-STAT1 antibody (M1407-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (M1407-1, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.