Desmin Mouse Monoclonal Antibody [3-F7]
cat.: M1501-10
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: 3-F7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within human Desmin aa 300-470.
Positive control: Rat heart tissue lysate, rat skeletal muscle tissue lysate, human skeletal muscle tissue lysate, human heart tissue lysate, D3, Hela, HepG2, human cervix tissue, human stomach cancer tissue, human uterus tissue.
Subcellular location: Cytopasm, Nucleus, Cell membrane
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:2,000
1:200
1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P17661 Human | P31001 Mouse | P48675 Rat
Alternative names: CMD1I CSM1 CSM2 DES DESM_HUMAN Desmin FLJ12025 FLJ39719 FLJ41013 FLJ41793 Intermediate filament protein OTTHUMP00000064865
Images
M1501-10_1.jpg Fig1: Western blot analysis of Desmin on different lysates with Mouse anti-Desmin antibody (M1501-10) at 1/1,000 dilution.

Lane 1: Rat heart tissue lysate
Lane 2: Rat skeletal muscle tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1501-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
M1501-10_2.jpg Fig2: Western blot analysis of Desmin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skeletal muscle tissue lysate, untreated
Lane 2: Human heart tissue lysate, untreated
M1501-10_3.jpg Fig3: All lanes: Western blot analysis of Desmin with anti-Desmin antibody (M1501-10) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2/3: Desmin knockout Hela whole cell lysate (10 µg).

M1501-10 was shown to specifically react with Desmin in wild-type Hela cells. NO bands were observed when Desmin knockout sample were tested. Wild-type and Desmin knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (M1501-10, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat anti-Mouse IgG-HRP antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature.
M1501-10_4.jpg Fig4: ICC staining Desmin in D3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Desmin monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.
M1501-10_5.jpg Fig5: ICC staining Desmin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Desmin monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
M1501-10_6.jpg Fig6: ICC staining Desmin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Desmin monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.
M1501-10_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Desmin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1501-10) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
M1501-10_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Desmin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1501-10) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
M1501-10_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1501-10) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
M1501-10_10.jpg Fig10: Flow cytometric analysis of Desmin was done on Hela cells. The cells were fixed, permeabilized and stained with Desmin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.