Desmin Recombinant Antibody [SI18-00] - Mouse IgG1 (Chimeric)
cat.: M1501-10
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC(Intra) |
| Clone number: | SI18-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Immunogen: | Synthetic peptide within Human Desmin aa 421-470 / 470. |
| Positive control: | RD (Human malignant embryonal rhabdomyosarcoma cell) cell lysate, C2C12 (Mouse myoblast) cell lysate, mouse heart tissue lysates, rat heart tissue lysates. |
| Subcellular location: | Cytopasm, Nucleus, Cell membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P FC(Intra) |
1:200,000 1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P17661 Human | P31001 Mouse | P48675 Rat |
| Alternative names: | CMD1I CSM1 CSM2 DES DESM_HUMAN Desmin FLJ12025 FLJ39719 FLJ41013 FLJ41793 Intermediate filament protein OTTHUMP00000064865 |
Images
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Fig1:
Western blot analysis of Desmin on different lysates with Mouse anti-Desmin antibody (M1501-10) at 1/200,000 dilution. Lane 1: RD (Human malignant embryonal rhabdomyosarcoma cell) cell lysate Lane 2: C2C12 (Mouse myoblast) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1501-10, 1/200,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 54 kDa |
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Fig2:
Western blot analysis of Desmin on mouse heart tissue lysate with Mouse anti-Desmin antibody (M1501-10) at 1/200,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1501-10, 1/200,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 54 kDa |
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Fig3:
Western blot analysis of Desmin on rat heart tissue lysate with Mouse anti-Desmin antibody (M1501-10) at 1/200,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1501-10, 1/200,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 54 kDa |
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Fig4:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: RD (Human malignant embryonal rhabdomyosarcoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: M1501-10, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: beta Tubulin (ET1602-4, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig5:
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: C2C12 (Mouse myoblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: M1501-10, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: beta Tubulin (ET1602-4, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig6:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Uterus (Smooth muscle) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: M1501-10, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: M1501-10, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig8:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: M1501-10, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig9:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: M1501-10, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig10:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Heart Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: M1501-10, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig11:
Application: Flow Cytometry (Intra) Species: Human Sample: RD (Human malignant embryonal rhabdomyosarcoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: M1501-10(1/1,000, Red) compared with Mouse IgG Isotype Control (HA601336, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature. |
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Fig12:
Application: Flow Cytometry (Intra) Species: Mouse Sample: C2C12 (Mouse myoblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: M1501-10(1/1,000, Red) compared with Mouse IgG Isotype Control (HA601336, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.