| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | F3-4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within human MASP2 aa 335-379/686. |
| Positive control: | Human serum |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: O00187 Human |
| Alternative names: | Mannan binding lectin serine peptidase 2 Mannan binding lectin serine protease 2 Mannose binding protein associated serine protease 2 MAP 19 MAP19 MASP 2 MBL associated plasma protein of 19 kD MBL associated serine protease 2 Small MBL associated protein sMAP |
|
Fig1:
Western blot analysis of MASP2 on human plasma lysate with Mouse anti-MASP2 antibody (M1501-11) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1501-11, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Anti-mouse IgG for IP Nano-secondary antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 75.7 kDa Observed band size: 76 kDa |