MASP2 Mouse Monoclonal Antibody [F3-4]
cat.: M1501-11
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: F3-4
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 76 kDa
Isotype: IgG2b
Immunogen: Synthetic peptide within human MASP2 aa 335-379/686.
Positive control: Human serum
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:2,000-1:5,000
Uniprot #: SwissProt: O00187 Human
Alternative names: Mannan binding lectin serine peptidase 2 Mannan binding lectin serine protease 2 Mannose binding protein associated serine protease 2 MAP 19 MAP19 MASP 2 MBL associated plasma protein of 19 kD MBL associated serine protease 2 Small MBL associated protein sMAP
Images
M1501-11_1.jpg Fig1: Western blot analysis of MASP2 on human plasma lysate with Mouse anti-MASP2 antibody (M1501-11) at 1/1,000 dilution.

Lysates/proteins at 30 µg/Lane.
Exposure time: 59 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1501-11, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Anti-mouse IgG for IP Nano-secondary antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 75.7 kDa
Observed band size: 76 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.