FGFR2/CD332 Mouse Monoclonal Antibody [1-F1-E7]
cat.: M1501-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 1-F1-E7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: ~100kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human FGFR2 / CD332 aa 601-723 / 821. |
| Positive control: | K562 cell lysates, MCF-7, Hela. |
| Subcellular location: | Cell membrane; Golgi apparatus |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:50 1:500-1:1,000 |
| Uniprot #: | SwissProt: P21802 Human | P21803 Mouse |
| Alternative names: | bacteria-expressed kinase BBDS BEK BEK fibroblast growth factor receptor BFR1 CD332 CD332 antigen CEK3 CFD1 Craniofacial dysostosis 1 ECT1 FGF receptor FGFR 2 FGFR-2 Fgfr2 FGFR2_HUMAN Fibroblast growth factor receptor 2 Hydroxyaryl protein kinase Jackson Weiss syndrome JWS K SAM K-sam Keratinocyte growth factor receptor 2 Keratinocyte growth factor receptor KGFR KSAM protein tyrosine kinase, receptor like 14 soluble FGFR4 variant 4 TK14 TK25 |
Images
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Fig1: Western blot analysis on K562 cell lysates using anti-FGFR2 mouse mAb. |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling FGFR2/CD332 with Mouse anti-FGFR2/CD332 antibody (M1501-2) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FGFR2/CD332 antibody (M1501-2) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Flow cytometric analysis of Hela cells labeling FGFR2/CD332. Cells were fixed and permeabilized.Then stained with the primary antibody (M1501-2, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a FITC conjugate-Goat anti-Mouse IgG Secondary antibody (HA1128) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.