JAK2 Mouse Monoclonal Antibody [6-D3]
cat.: M1501-8
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 6-D3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: ~130kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human JAK2 aa 883-1,132 / 1,132. |
| Positive control: | K562, Hela, human breast carcinoma tissue |
| Subcellular location: | Cytopasm, nucleus |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:2,000 1:50 1:200 1:200 |
| Uniprot #: | SwissProt: O60674 Human |
| Alternative names: | JAK 2 JAK-2 JAK2 JAK2_HUMAN Janus Activating Kinase 2 Janus kinase 2 (a protein tyrosine kinase) Janus kinase 2 JTK 10 JTK10 kinase Jak2 OTTHUMP00000043260 THCYT3 Tyrosine protein kinase JAK2 Tyrosine-protein kinase JAK2 |
Images
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Fig1: Western blot analysis on K562 cell lysates using anti-JAK2 mouse mAb. |
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Fig2:
Western blot analysis of JAK2 on different lysates with Mouse anti-JAK2 antibody (M1501-8) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si JAK2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1501-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining JAK2 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti- JAK2 mouse mAb. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.