EEA1 Mouse Monoclonal Antibody [B0-A8]
cat.: M1503-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | B0-A8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 162 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide of the N terminal residues of Human EEA1. |
| Positive control: | HeLa cell lysate, Daudi cell lysate, A431 cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human prostate tissue, mouse prostate tissue. |
| Subcellular location: | Cytoplasm, early endosome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:10,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q15075 Human | Q8BL66 Mouse | A0A0G2K051 Rat |
| Alternative names: | Early endosome antigen 1 Early endosome antigen 1, 162kD Early endosome associated protein EEA 1 EEA1 EEA1_HUMAN Endosome associated protein p162 Endosome-associated protein p162 MST105 MSTP105 ZFYVE2 Zinc finger FYVE domain containing protein 2 Zinc finger FYVE domain-containing protein 2 |
Images
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Fig1:
Western blot analysis of EEA1 on different lysates with Mouse anti-EEA1 antibody (M1503-1) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-EEA1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1503-1) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of EEA1 on different lysates with Mouse anti-EEA1 antibody (M1503-1) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: A431 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1503-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-EEA1 antibody (M1503-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1503-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-EEA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1503-1) at 1/400 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.