ERAS Mouse Monoclonal Antibody [D10-B9]
cat.: M1505-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | D10-B9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human ERAS aa 1-233 / 233. |
| Positive control: | NCCIT, human liver carcinoma tissue, human breast carcinoma tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell IHC-P |
1:50 1:200-1:500 |
| Uniprot #: | SwissProt: Q7Z444 Human |
| Alternative names: | E ras E-Ras ecat5 Embryonic stem cell expressed Ras Embryonic stem cell-expressed Ras Eras ES cell expressed Ras GTPase ERas Ha-Ras2 HRAS2 HRASP MGC126691 MGC126693 OTTHUMP00000065857 RASE_HUMAN Small GTPase protein E Ras v Ha ras Harvey rat sarcoma viral oncogene homolog pseudogene v Ha ras Harvey rat sarcoma viral oncogene homolog 2 |
Images
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Fig1: ICC staining ERAS in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ERAS monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-ERAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1505-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1505-1) at 1/400 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.