Lamin B2 Mouse Monoclonal Antibody [J9-C4]
cat.: M1505-10
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | J9-C4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Lamin B2 aa 1-50 / 620. |
| Positive control: | Jurkat cell lysates, human lung cancer tissue. |
| Subcellular location: | Nucleus lamina. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q03252 Human |
| Alternative names: | LAMB 2 LAMB2 Lamin-B2 LMN 2 LMN B2 LMN2 LMNB 2 LMNB2 LMNB2_HUMAN MGC2721 RGD1563803 |
Images
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Fig1: Western blot analysis on Jurkat cell lysates using anti- LMNB2 mouse mAb. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-LMNB2 antibody (M1505-10) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-10) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.