Lamin B2 Mouse Monoclonal Antibody [D5-C2]
cat.: M1505-11
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | D5-C2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within Human Lamin B2 aa 571-620 / 620. |
| Positive control: | Jurkat cell lysates, hybrid fish (crucian-carp) brain tissue lysates, human uterine tissue. |
| Subcellular location: | Nucleus inner membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:200-1:500 1:500-1:1000 |
| Uniprot #: | SwissProt: Q03252 Human | Q62733 Rat | P21619 Mouse | B3DFN3 Zebrafish |
| Alternative names: | Lamin-B2 Lamin B2 LMN 2 LMN B2 LMN2 LMNB 2 LMNB2 LMNB2_HUMAN MGC2721 RGD1563803 |
Images
|
Fig1: Western blot analysis of Lamin B2 on Jurkat cell lysate using anti-Lamin B2 antibody at 1/500 dilution. |
|
Fig2: Western blot analysis of Lamin B2 on zebrafish tissue lysate using anti-Lamin B2 antibody at 1/500 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human uterine tissue with Mouse anti-Lamin B2 antibody (M1505-11) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-11) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.