Lamin B2 Mouse Monoclonal Antibody [D5-C2]
cat.: M1505-11
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | D5-C2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within Human Lamin B2 aa 571-620 / 620. |
| Positive control: | Hela cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, human uterine tissue. |
| Subcellular location: | Nucleus inner membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:200-1:500 1:500-1:1000 |
| Uniprot #: | SwissProt: Q03252 Human | Q62733 Rat | P21619 Mouse | B3DFN3 Zebrafish |
| Alternative names: | Lamin-B2 Lamin B2 LMN 2 LMN B2 LMN2 LMNB 2 LMNB2 LMNB2_HUMAN MGC2721 RGD1563803 |
Images
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Fig1:
Western blot analysis of Lamin B2 on different lysates with Mouse anti-Lamin B2 antibody (M1505-11) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1505-11, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 70 kDa Observed band size: 70 kDa |
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Fig2: Western blot analysis of Lamin B2 on zebrafish tissue lysate using anti-Lamin B2 antibody at 1/500 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human uterine tissue with Mouse anti-Lamin B2 antibody (M1505-11) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-11) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.