Cdk4 Mouse Monoclonal Antibody [5-C3-G3]
cat.: M1505-5
| Product Type: | Mouse monoclonal IgG2c, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 5-C3-G3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG2c |
| Immunogen: | Recombinant protein within human Cdk4 aa 80-303/303. |
| Positive control: | A549 cell lysate, 293T cell lysate, HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate, mouse lung tissue lysate, human breast cancer tissue, human stomach cancer tissue, human thyroid cancer tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Nucleus membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P11802 Human | P30285 Mouse |
| Alternative names: | Cdk 4 cdk4 CDK4 protein CDK4_HUMAN Cell division kinase 4 Cell division protein kinase 4 CMM 3 CMM3 Crk3 Cyclin dependent kinase 4 Cyclin-dependent kinase 4 Melanoma cutaneous malignant 3 MGC14458 p34 cdk4 PSK J3 PSK-J3 |
Images
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Fig1:
Western blot analysis of Cdk4 on different lysates with Mouse anti-Cdk4 antibody (M1505-5) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1505-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cdk4 antibody (M1505-5) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-5) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Cdk4 antibody (M1505-5) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-5) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Mouse anti-Cdk4 antibody (M1505-5) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-5) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.