M1505-8

Product Type:	Mouse monoclonal IgM, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Monoclonal
Clone number:	A2-7

Form:

Liquid

Storage condition:

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2ug/ul

Purification:

Protein L affinity purified.

Molecular weight:

50 kDa

Isotype:

IgM

Immunogen:

Synthetic peptide within Human NFKB1 aa 317-366 / 968.

Positive control:

Mouse spleen tissue lysate, Rat spleen tissue lysate, THP-1 cell lysate, HepG2, human breast carcinoma tissue

Subcellular location:

Nucleus. Cytoplasm.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000-1:2,000
1:200
1:200-1:500

Uniprot #:

SwissProt: P19838 Human

Alternative names:

DKFZp686C01211 DNA binding factor KBF1 DNA binding factor KBF1 EBP1 DNA-binding factor KBF1 EBP 1 EBP-1 EBP1 KBF1 MGC54151 NF kappa B NF kappaB NF kappabeta NF kB1 NFkappaB NFKB 1 NFKB p105 NFKB p50 Nfkb1 NFKB1_HUMAN Nuclear factor kappa B DNA binding subunit Nuclear factor kappa-B, subunit 1 Nuclear factor NF kappa B p105 subunit Nuclear factor NF kappa B p50 subunit Nuclear factor NF-kappa-B p50 subunit Nuclear factor of kappa light chain gene enhancer in B cells 1 Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 p105 p50 p84/NF-kappa-B1 p98 Transcription factor NFKB1

	Fig1: Western blot analysis of NF-kB p105 / p50 on different lysates with Mouse anti-NF-kB p105 / p50 antibody (M1505-8) at 1/2,000 dilution. Lane 1: Mouse spleen tissue lysate (40 ug/lane) Lane 2: Rat spleen tissue lysate (40 ug/lane) Lane 3: THP-1 (Human acute monoblastic leukemia cell) cell lysate (20 ug/lane) Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1505-8, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa
	Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 antibody. Counter stained with hematoxylin.
	Fig3: ICC staining NF-κB p105/p50 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.