Product Type: | Mouse monoclonal IgM, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Monoclonal |
Clone number: | A2-7 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein L affinity purified. |
Molecular weight: | 50 kDa |
Isotype: | IgM |
Immunogen: | Synthetic peptide within Human NFKB1 aa 317-366 / 968. |
Positive control: | MCF-7, HepG2, human breast carcinoma tissue |
Subcellular location: | Nucleus. Cytoplasm. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:200 1:200-1:500 |
Uniprot #: | SwissProt: P19838 Human |
Alternative names: | DKFZp686C01211 DNA binding factor KBF1 DNA binding factor KBF1 EBP1 DNA-binding factor KBF1 EBP 1 EBP-1 EBP1 KBF1 MGC54151 NF kappa B NF kappaB NF kappabeta NF kB1 NFkappaB NFKB 1 NFKB p105 NFKB p50 Nfkb1 NFKB1_HUMAN Nuclear factor kappa B DNA binding subunit Nuclear factor kappa-B, subunit 1 Nuclear factor NF kappa B p105 subunit Nuclear factor NF kappa B p50 subunit Nuclear factor NF-kappa-B p50 subunit Nuclear factor of kappa light chain gene enhancer in B cells 1 Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 p105 p50 p84/NF-kappa-B1 p98 Transcription factor NFKB1 |
Fig1: Western blot analysis of NF-κB p105/p50 on MCF-7 cell lysates. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 antibody. Counter stained with hematoxylin. | |
Fig3: ICC staining NF-κB p105/p50 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |