Cathepsin B Mouse Monoclonal Antibody [J11-A11]
cat.: M1506-1
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Zebrafish, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: J11-A11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within human Cathepsin B aa 300-338/338.
Positive control: RAW264.7 cell lysate, PC-12 cell lysate, SW480 cell lysates, SW480.
Subcellular location: Lysosome, Melanosome, Secreted, Apical cell membrane.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:50
Uniprot #: SwissProt: P07858 Human | P10605 Mouse | P00787 Rat
Alternative names: Amyloid precursor protein secretase APP secretase APPS CATB_HUMAN Cathepsin B heavy chain Cathepsin B1 CathepsinB CPSB CTSB cysteine protease OTTHUMP00000116009 OTTHUMP00000229510 OTTHUMP00000229511 OTTHUMP00000229512 OTTHUMP00000229514 OTTHUMP00000229515 OTTHUMP00000229516 Preprocathepsin B
Images
M1506-1_1.jpg Fig1: Western blot analysis of Cathepsin B on different lysates with Mouse anti-Cathepsin B antibody (M1506-1) at 1/1,000 dilution.

Lane 1: RAW264.7 cell lysate
Lane 2: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 38 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
M1506-1_2.jpg Fig2: Western blot analysis of Cathepsin B on SW480 cell lysate using anti-Cathepsin B antibody at 1/5,000 dilution.



M1506-1_3.jpg Fig3: Immunocytochemistry analysis of SW480 cells labeling Cathepsin B with Mouse anti-Cathepsin B antibody (M1506-1) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cathepsin B antibody (M1506-1) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.