Cathepsin B Mouse Monoclonal Antibody [J11-A11]
cat.: M1506-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Zebrafish, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | J11-A11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Cathepsin B aa 300-338/338. |
| Positive control: | RAW264.7 cell lysate, PC-12 cell lysate, SW480 cell lysates, SW480. |
| Subcellular location: | Lysosome, Melanosome, Secreted, Apical cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50 |
| Uniprot #: | SwissProt: P07858 Human | P10605 Mouse | P00787 Rat |
| Alternative names: | Amyloid precursor protein secretase APP secretase APPS CATB_HUMAN Cathepsin B heavy chain Cathepsin B1 CathepsinB CPSB CTSB cysteine protease OTTHUMP00000116009 OTTHUMP00000229510 OTTHUMP00000229511 OTTHUMP00000229512 OTTHUMP00000229514 OTTHUMP00000229515 OTTHUMP00000229516 Preprocathepsin B |
Images
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Fig1:
Western blot analysis of Cathepsin B on different lysates with Mouse anti-Cathepsin B antibody (M1506-1) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Cathepsin B on SW480 cell lysate using anti-Cathepsin B antibody at 1/5,000 dilution. |
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Fig3:
Immunocytochemistry analysis of SW480 cells labeling Cathepsin B with Mouse anti-Cathepsin B antibody (M1506-1) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cathepsin B antibody (M1506-1) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.