SIRT1 Mouse Monoclonal Antibody [7-C5-B2]
cat.: M1506-3
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7-C5-B2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within Human SIRT1 aa 698-747 / 747. |
| Positive control: | HAP1 cell lysate, HeLa, human lung carcinoma tissue, human colon carcinoma tissue |
| Subcellular location: | Nucleus, PML body, Cytoplasm. |
| Recommended Dilutions:
IHC-P IF-Cell WB |
1:200 1:100 1:2,000 |
| Uniprot #: | SwissProt: Q96EB6 Human |
| Alternative names: | 75SirT1 hSIR2 hSIRT1 HST2, S. cerevisiae, homolog of NAD dependent deacetylase sirtuin 1 NAD dependent protein deacetylase sirtuin 1 OTTHUMP00000198111 OTTHUMP00000198112 Regulatory protein SIR2 homolog 1 SIR1_HUMAN SIR2 SIR2 like 1 SIR2 like protein 1 SIR2, S.cerevisiae, homolog-like 1 SIR2-like protein 1 SIR2ALPHA SIR2L1 Sirt1 SirtT1 75 kDa fragment Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) Sirtuin 1 Sirtuin type 1 |
Images
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Fig1:
Western blot analysis of SIRT1 on different lysates with Mouse anti-SIRT1 antibody (M1506-3) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SIRT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 120 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1506-3) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling SIRT1 with Mouse anti-SIRT1 antibody (M1506-3) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-SIRT1 antibody (M1506-3) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- SIRT1 mouse mAb. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti- SIRT1 mouse mAb. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.