C19orf35 Mouse Monoclonal Antibody [3-B7]
cat.: M1506-5
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 3-B7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human C19orf35 aa 1-473 / 473. |
| Positive control: | Human testis tissue, Hela, hES cells |
| Subcellular location: | Actin cytoskeleton, focal adhesion. |
| Recommended Dilutions:
IHC-P IF-Cell |
1:200-1:500 1:200 |
| Uniprot #: | SwissProt: Q6ZS72 Human |
| Alternative names: | C19orf35 chromosome 19 open reading frame 35 CS035_HUMAN FLJ45778 Putative uncharacterized protein C19orf35 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-C19orf35 antibody (M1506-5) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1506-5) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: ICC staining C19orf35 in human embryonic stem cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining C19orf35 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.