Cyclin B1 Mouse Monoclonal Antibody [C2-F2]
cat.: M1508-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | C2-F2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide (KLH-coupled) within human Cyclin B1 aa 391-433. |
| Positive control: | K562 cell lysates, HepG2, LOVO. |
| Subcellular location: | Cytoplasm, Nucleus, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:200-1:500 |
| Uniprot #: | SwissProt: P14635 Human |
| Alternative names: | CCNB 1 CCNB ccnb1 CCNB1_HUMAN Cyclin B1 G2 mitotic specific cyclin B1 G2/mitotic-specific cyclin-B1 |
Images
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Fig1:
Western blot analysis of Cyclin B1 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 58 kDa |
|
Fig2: ICC staining Cyclin B1 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. |
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Fig3: ICC staining Cyclin B1 in LOVO cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin B1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.