ALDH2 Mouse Monoclonal Antibody [E4-D10]
cat.: M1509-1
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: E4-D10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human ALDH2 aa 468-517 / 517.
Positive control: HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, SK-Br-3, HeLa, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human gastric cancer tissue, mouse colon tissue, mouse stomach tissue.
Subcellular location: Mitochondrion matrix.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000-1:5,000
1:100
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P05091 Human | P47738 Mouse | P11884 Rat
Alternative names: Acetaldehyde dehydrogenase 2 Aldehyde dehydrogenase 2 family (mitochondrial) Aldehyde dehydrogenase 2 family Aldehyde dehydrogenase mitochondrial Aldehyde dehydrogenase, mitochondrial ALDH 2 ALDH class 2 ALDH E2 ALDH-E2 Aldh2 ALDH2_HUMAN ALDHI ALDM Liver mitochondrial ALDH MGC1806 Mitochondrial aldehyde dehydrogenase 2 MS767 Nucleus encoded mitochondrial aldehyde dehydrogenase 2
Images
M1509-1_1.jpg Fig1: Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (M1509-1) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 56 kDa
Observed band size: 50 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1509-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
M1509-1_2.jpg Fig2: Immunocytochemistry analysis of SK-Br-3 cells labeling ALDH2 with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
M1509-1_3.jpg Fig3: Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (M1509-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
M1509-1_4.jpg Fig4: Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-ALDH2 Mouse mAb.
M1509-1_5.jpg Fig5: Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-ALDH2 Mouse mAb.
M1509-1_6.jpg Fig6: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-ALDH2 Mouse mAb.
M1509-1_7.jpg Fig7: Immunohistochemical analysis of paraffin- embedded human gastric cancer tissue using anti-ALDH2 Mouse mAb.
M1509-1_8.jpg Fig8: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-ALDH2 Mouse mAb.
M1509-1_9.jpg Fig9: Immunohistochemical analysis of paraffin- embedded mouse stomach tissue using anti-ALDH2 Mouse mAb.
M1509-1_10.jpg Fig10: Flow cytometric analysis of HepG2 cells with ALDH2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.