AK6 Mouse Monoclonal Antibody [B1-F4]
cat.: M1509-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | B1-F4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human AK6 aa 123-172 / 172. |
| Positive control: | K-562 (Human chronic myelogenous leukemia cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, MCF-7. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y3D8 Human |
| Alternative names: | AD-004 Adenylate kinase isoenzyme 6 Adrenal gland protein AD-004 AK/ATPase AK6 CGI-137 CIP Coilin-interacting nuclear ATPase protein Dual activity adenylate kinase/ATPase hCINAP KAD6_HUMAN TAF9 |
Images
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Fig1:
Western blot analysis of AK6 on different lysates with Mouse anti-AK6 antibody (M1509-2) at 1/2,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 4: MCF7 (Human breast cancer cell) cell lysate Lane 5: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1509-2, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 20 kDa |
|
Fig2: ICC staining AK6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.