ESD Mouse Monoclonal Antibody [J0-8]
cat.: M1510-1
| Product Type: | Mouse monoclonal IgG2c, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | J0-8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG2c |
| Immunogen: | Recombinant protein within Human ESD aa 1-182 / 282. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse colon tissue lysate, mouse stomach tissue lysate, rat kidney tissue lysate, rat colon tissue lysate, rat stomach tissue lysate, Hela, HepG2, SW480, human tonsil tissue, human kidney tissue, Jurkat. |
| Subcellular location: | Cytoplasm. Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:20,000 1:100 1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P10768 Human | Q9R0P3 Mouse | B0BNE5 Rat |
| Alternative names: | EC 3.1.2.12 Es-10 Es10 ESD ESTD_HUMAN Esterase 10 Esterase D Esterase D formylglutathione hydrolase FGH FGH, included FLJ11763 Methylumbelliferyl acetate deacetylase MGC139609 OTTHUMP00000018374 OTTHUMP00000040929 S formylglutathione hydrolase S-formylglutathione hydrolase S-formylglutathione hydrolase, included Sid 478 sid478 |
Images
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Fig1:
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (M1510-1) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: SW480 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (M1510-1) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Mouse stomach tissue lysate Lane 5: Rat kidney tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of ESD on different lysates with Rabbit anti-ESD antibody (M1510-1) at 1/20,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ESD KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-1) at 1/20,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4: ICC staining ESD in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ESD monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining ESD in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ESD monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig6: ICC staining ESD in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ESD monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ESD antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ESD antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-1) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig9: Flow cytometric analysis of ESD was done on Jurkat cells. The cells were fixed, permeabilized and stained with ESD antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.