| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP, ELISA |
| Clonality: | Monoclonal |
| Clone number: | E0-G7 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide (YTDIEMNRLGKC). |
| Positive control: | Recombinant VSV-G-tag protein. |
| Recommended Dilutions:
WB |
1:1,000-1:20,000 |
| Alternative names: | Vesicular stomatitis virus glycoprotein tag VSV epitope tag VSV tag VSV-G epitope tag VSVG vsvg tag |
|
Fig1:
Western blot analysis of VSV-G-tag on recombinant VSV-G-tag protein with Mouse anti-VSV-G-tag antibody (M1510-10) at different dilutions. Lysates/proteins at 50 ng/Lane. Exposure time: 15 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-10) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |