Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Species independent |
Applications: | WB, IP, ELISA |
Clonality: | Monoclonal |
Clone number: | E0-G7 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide. |
Positive control: | Recombinant VSV-G-tag protein. |
Recommended Dilutions:
WB |
1:1,000-1:20,000 |
Alternative names: | Vesicular stomatitis virus glycoprotein tag VSV epitope tag VSV tag VSV-G epitope tag VSVG vsvg tag |
Fig1:
Western blot analysis of VSV-G-tag on recombinant VSV-G-tag protein with Mouse anti-VSV-G-tag antibody (M1510-10) at different dilutions. Lysates/proteins at 50 ng/Lane. Exposure time: 15 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-10) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |