Fibrinogen Mouse Monoclonal Antibody [D5-B3]
cat.: M1510-11
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | D5-B3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 95/56/52 kDa |
| Isotype: | IgG1 |
| Immunogen: | native protein. |
| Positive control: | HepG2 cell lysates, human liver tissue, human placenta tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P WB |
1:50-1:200 1:500-1:2000 |
| Uniprot #: | SwissProt: P02671 Human | P02675 Human | P02679 Human |
| Alternative names: | FGA Fib2 FIBA_HUMAN Fibrinogen A alpha polypeptide Fibrinogen alpha chain Fibrinogen B alpha polypeptide Fibrinogen beta chain Fibrinogen G alpha polypeptide Fibrinogen gamma chain fibrinogen, B beta polypeptide fibrinogen, G gamma polypeptide fibrinogen, gamma polypeptide Fibrinogen--alpha -polypeptide chain Fibrinogen--beta -polypeptide chain Fibrinogen--gamma-polypeptide chain |
Images
|
Fig1: Western blot analysis of Fibrinogen on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-11, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.