Transferrin Mouse Monoclonal Antibody [A11-A3]
cat.: M1510-15
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A11-A3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG1 |
| Immunogen: | Native protein. |
| Positive control: | Human liver tissue, human kidney tissue, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P FC |
1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P02787 Human |
| Alternative names: | Apotransferrin Beta 1 metal binding globulin Beta-1 metal-binding globulin DKFZp781D0156 PRO1400 PRO1557 PRO2086 Serotransferrin Serotransferrin precursor Siderophilin TF TFQTL1 Transferin Transferrin TRFE_HUMAN |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-15) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-15) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
|
Fig3: Flow cytometric analysis of Transferrin was done on HepG2 cells. The cells were fixed, permeabilized and stained with Transferrin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.