Transferrin Mouse Monoclonal Antibody [E6-E10]
cat.: M1510-19
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Clonality: | Monoclonal |
| Clone number: | E6-E10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG1 |
| Immunogen: | Native protein. |
| Positive control: | Recombinant protein, human liver tissue, human kidney tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P ELISA |
1:500 1:600 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02787 Human |
| Alternative names: | Apotransferrin Beta 1 metal binding globulin Beta-1 metal-binding globulin DKFZp781D0156 PRO1400 PRO1557 PRO2086 Serotransferrin Serotransferrin precursor Siderophilin TF TFQTL1 Transferin Transferrin TRFE_HUMAN |
Images
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Fig1:
Western blot analysis of Transferrin on Recombinant protein with Mouse anti-Transferrin antibody (M1510-19) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 77 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-19) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Transferrin antibody (M1510-19) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-19) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Transferrin antibody (M1510-19) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-19) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.