Alpha-2-macroglobulin Mouse Monoclonal Antibody [3-G5]
cat.: M1510-21
| Product Type: | Mouse monoclonal IgG2c, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, ELISA |
| Clonality: | Monoclonal |
| Clone number: | 3-G5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 163 kDa |
| Isotype: | IgG2c |
| Immunogen: | Recombinant protein within human Alpha-2-macroglobulin aa 950-1200. |
| Positive control: | Human liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P ELISA |
1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01023 Human |
| Alternative names: | A2m A2MG_HUMAN Alpha 2 M Alpha 2M Alpha-2-M Alpha-2-macroglobulin C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 CPAMD5 DKFZp779B086 FWP007 S863 7 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alpha-2-macroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-21, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.