Alpha-2-macroglobulin Mouse Monoclonal Antibody [B1-D7]
cat.: M1510-24
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, ELISA
Clonality: Monoclonal
Clone number: B1-D7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 163 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.
Positive control: LO2 cell lysates, human endometrium tissue, human testis tissue, human liver tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P
  ELISA

1:500-1:2,000
1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P01023 Human
Alternative names: A2m A2MG_HUMAN Alpha 2 M Alpha 2M Alpha-2-M Alpha-2-macroglobulin C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 CPAMD5 DKFZp779B086 FWP007 S863 7
Images
M1510-24_1.jpg Fig1: Western blot analysis of Alpha-2-macroglobulin on LO2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
M1510-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Mouse anti-Alpha-2-macroglobulin antibody (M1510-24) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1510-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Alpha-2-macroglobulin antibody (M1510-24) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1510-24_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Alpha-2-macroglobulin antibody (M1510-24) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-24) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.